CRMAGE
Tool for magic!
Please cite:
Ronda C, Pedersen LE et al
CRMAGE: CRISPR Optimized MAGE Recombineering
Scientific Reports 6, Article number: 19452 (2016)
doi:10.1038/srep19452

Step 1 - - Enter Sequence

Next step...

Step 2 - Select base to mutate

Selected base:
Click a base to select it..

What should we change it to?
Next step...

Step 3 - Select targets and silent mutations

Select the correct frame for the oligo below?

Region around selected base with single letter amino acids

A target region will appear here
Available Targets in selected region. Select desired silent mutations in the drop down box(es).
A list of targets will appear here

This is the final sequence. It is updated when you change some of the above.

Final sequence will appear here by magic
Technical University of Denmark Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark

Lasse Ebdrup Pedersen
Novo Nordisk Foundation Center for Biosustainability
Technical University of Denmark